In this study authors aim to determine the effect of taurine in a rat model of diet-induced NAFLD, focusing in the role of sterol regulatory element-binding proteins (SREBP1/2) and adenine monophosphate (AMP) activated protein kinase (AMPK) as possible targets of this compound.
Please find below my comments:
- I understand that it is out of the scope of the research, but do you think that intestinal microbiota may be contributing to the observed effects of taurine administration?
- Some grammar and spelling errors need to be corrected. I.e: page 4 line 12 “acid amino”
- In our experience animals under HFD tends to eat similar or even less than control diet fed counterparts, do you have an explanation for the significant higher food intake of HFD-fed rats in your study?
- Maybe table 1 as well as figures 1 and 2 could be allocated to supplementary material, in order to comply with the maximum figures/tables established in the guidelines.

I want to thank the authors for their kind respond to my comments and their effort to include the information that I requested in the paper.