Title: ''Prevalence and incidence of vector‑borne pathogens in unprotected dogs in two Brazilian regions''

Comment: the title is informative and relevant.

Abstract section: 1-a-'' Herein, we assessed the prevalence and the year-crude incidence (YCI) of Leishmania spp. and other VBPs in privately-owned dogs from two geographical regions of Brazil''.

b-'' We confirmed a high prevalence and YCI of various VBPs among privately-owned dogs in two geo graphical regions of Brazil''.

c-'' Our data also indicate that the risk of infection varies significantly for individual VBPs and between the regions, which may be related to several factors that are still poorly understood''.

Comment: In English academic writing, it is important to avoid personal bias. Using “I” or “we” makes the essay about you and your experiences, instead of research and concrete details. ... This is not okay in formal writing, especially essays or research papers but it is okay when writing a personal letter, so the correct sentences suitable to be: a- ''It has been assessed the prevalence and the year………………''. b-''This study has confirmed a high prevalence and ………………..''

c-''The results of this study has also indicated that the risk…………..''.

Background section:

2-''In this context, we estimated the year-crude incidence (YCI) of infection by Leishmania spp. in dogs from two municipalities of Brazil''.

Comment: as English academic writing, the sentence should be written ''In this study, it has been estimated the year ………….''

Methods section: Statistical analysis

3- '' Likewise, we used the last observation carried forward (LOCF) method, so data from dogs tested at the interim follow-up, but removed before the final follow-up were included in the calculations''

Comment: In academic writing, it's important to avoid personal bias. Using “I” or “we” makes the essay about you and your experiences, instead of research and concrete details. So the correct sentences suitable to be: ''Likewise, it has been used the last observation………………..''

Results and discussion Section

4-a-'' Our previous study with a much smaller sample size also suggested a higher prevalence of Leishmania spp. '' b-'' This is also in line with our previous study, in which we reported high seropositivities for Babesia spp. in both Goiana and São Joaquim de Bicas'' c-'' our results may suggest that the tick population present in Goiana may be more capable of transmitting E. canis as compared to the population of São Joaquim de Bicas''

Comment: In academic writing, it's important to avoid personal bias. Using “I” or “we” makes the essay about you and your experiences, instead of research and concrete details. So the correct sentences suitable to be as follow:- a:-''In the previous study with…………………………….''

b:-''………..with the previous study that has been reported …………''

c:-''The results of this study were suggested that ………''

Conclusion section 5-:'' Our data also indicate that the risk of infection varies signifcantly for individual pathogens and between the regions, which may be related to several biotic'' Comment: the correct sentences suitable to be as follow: ''The data in this study also indicate that ……………..….''