The general impression is that the standard of written English need to be improved, there were numerous grammatical errors and poor sentence construction throughout the manuscript.
The methods section should come after introduction and not after the discussion.
There is confusion as to the actual number of rats used in this study. In the abstract section it was stated that 32 adult male Wister rats were used. They were divided into four groups: Control, Control + TDZD-8, CIA, and CIA + TDZD-8. This would mean that there were 8 rats per group but it was listed as n=18/group in the abstract and experimental design section. However, analysis of data as per results section was based on 6/group. The actual numbers of rats used just do not add up.
This study is similar to that of Yong-Jin Kwon, Chong-Hyeon Yoon, Sang-Won Lee, Yong-Beom Park, Soo-Kon Lee, Min-Chan Park, Inhibition of glycogen synthase kinase-3 suppresses inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes and collagen-induced arthritis, Joint Bone Spine, 2014; 81: 240-246. This paper was not cited in the bibliography and the authors are strongly recommended to read the full text of this published paper. This paper not only carry out in vitro and in vivo studies but also compare CIA with RA patients.

There is no new information nor novelty to fill existing knowledge gaps on the subject matter in this manuscript.

There is marked improvement in the revised manuscript. Comments from previous reviewers were deleted and rewritten. However the standard of written English could be further improved. The methods section should come after introduction. The sequence of presentation is counter to that listed under author guide.
It is much clearer now as to how many rats were used in each experiments. That TDZD-8 inhibits the cross-talk between RA, ERS, and autophagy is not convincing enough. TDZD-8 has been used to study the role of GSK3β during stem cell differentiation as well as in cell and animal models of Alzheimer’s disease and other neurodegenerative disorders.
I still could not see any new information nor novelty to fill existing knowledge gaps on the subject matter in this manuscript.