Comments on abstract, title, references

The title of this manuscript is concise and appropriate. This is a report about a newly isolated Cyclohexylamine oxidase (CHAO) from Acinetobacter sp. YT-02. It is a significant contribution to the biodegradation field. The authors have characterized the CHAO in details. The reference list covers the relevant literature adequately and in an unbiased manner.

Comments on introduction/background

Introduction section of the manuscript is very small. I recommend authors to elaborate little bit to retain the balance of the overall manuscript. The English language of the manuscript has sufficient quality for publication.

Comments on methodology

The methods used by the authors are standard and allows replication studies. The authors have isolated and cloned the CHAO. They have found only 48% sequence similarity between the previously reported work. I recommend authors to opt NMR or another crystallographic method elucidate the enzyme structure, only homology modeling is not enough.

Comments on data and results

This is a report about a newly isolated Cyclohexylamine oxidase from Acinetobacter sp. YT02. It includes molecular cloning, enzyme kinetics, and basic bioinformatic analysis. The quality of the table is satisfactory. However, the use of extra space with a black outline is not a good way for figure presentation. I recommend authors re-check their figure presentation style. For example, the authors should enlarge figures and merge line diagrams where possible.

Comments on discussion and conclusions

The discussion section of the manuscript is small. I recommend the authors rewrite this particular section with little depth manner, a generic description, and comparisons of previous results are not helpful in this context. For examples, the inhibitory effect of different metal ions should be articulated in-depth manner.