In this study, a drug-loaded nano-drug delivery system was independently developed, which effectively addressed the challenge of drug delivery for Rm following spinal cord injury (SCI). This system significantly increased cAMP levels at the injury site, promoted neuronal survival, and demonstrated potential clinical relevance. However, several limitations remain to be addressed:
1. Although the nano-drug delivery system was employed, the drug release kinetics, including the duration and extent of release, were not quantitatively assessed.
2. In the SCI model, the behavioral changes following drug administration require further validation through systematic observation and testing.
3. The activity and electrophysiological conductivity of neurons should be verified using techniques such as electromyography (EMG), which were not conducted in the current study.