The is a very straight forward study by Watanabe and colleagues where they assessed the transcriptomic expression of critical receptors for the hormonal regulation of the reproductive axis: Ar, Esr1, Pgr. The studied this expression in Kiss1 and GABAergic neurons of the arcuate nucleus (ARC) or female mice across development. The main focus on females comes from the interest of this lab in PCOS. The study is very straight forward and well designed. Despite the simplicity, the results are quite relevant to understand the progression of the PCOS phenotype. Interestingly, while most Kiss1 ARC neurons express Ar, Esr1 and Pgr in adulthood, very few express Pgr in prepubertal ages. On the contrary, GABAergic neurons express the three receptors mRNA in all postnatal ages, although the expression increased with age. This expression of sex steroid receptors in Kiss1 and GABA neurons from the ARC suggests that these neurons are sensitive to circulating sex steorids from early in life and therefore sensitive to regulation exerted by factors that may determine the onset of PCOS. The study is well designed and the manuscript well written. There are no major comments.
Minor comments:
- Describe the animal section better. What breedings were setup? Was litter sized controlled? This may affect the expression of genes at early ages.
- Line 118: Diestrous vs diestrus.

The authors have successfully addressed all of my comments.