In the manuscript entitled “Synthesis, enzyme inhibition assay, and molecular modeling study of novel pyrazolines linked to 4-methylsulfonylphenyl scaffold: Antitumor activity and cell cycle analysis” by Ayyad and co-workers, the authors have synthesized a series of pyrazoline-linked 4-methylsulfonylphenyl derivatives. These compounds were evaluated for their in vitro antitumor activity in different cancer cell lines and compared with reference drugs. The enzyme inhibitory potency of these compounds was investigated against EGFR, HER2, and VEGFR2 kinases. Molecular docking studies were performed to understand their binding interaction with the pyrazoline moiety. The synthesized compounds have been characterized by NMR, IR, Mass, and melting point. Overall, this is a significant finding, and the synthesized compound has the potential to serve as an attractive kinase inhibitor. Therefore, this reviewer recommends the manuscript for publication, provided the following issues are addressed:

1. The inhibition study was performed at a single dose. The authors should consider testing different lower concentrations to provide a more comprehensive understanding of the inhibition profile.

2. The manuscript is not well organized, particularly with respect to the placement of figures. Figures should be included in the main manuscript text rather than after the conclusion.

3. For novel compounds, the authors should provide High-Resolution Mass Spectrometry (HRMS) data for the synthesized compounds. Additionally, deuterated solvent peaks should be assigned in the NMR spectra