The study investigated, Ref: Submission ID: EJN-2024-03-31490 and “D-Limonene reduces depression-like behavior and enhances learning and memory through an anti-neuroinflammatory mechanism in male rats subjected to chronic restraint stress” titled I think it is a remarkable work. The authors aimed to investigate the effects of D-limonene, which has been suggested to have an anti-inflammatory effect, on long-term stress that causes chronic inflammation. Therefore, they evaluated the effects of D-limonene on brain hippocampal tissue and cognitive functions in male rats exposed to restraint stress by in vivo and biochemical studies. This research is acceptable considering the following recommendations.
1. No significance was noted in the graph abstract for the effect of D-limonene on the results of the sucrose preference test in male rats exposed to chronic restraint stress shown in the graphical abstract. I recommend you add it.
2. Which product of Fluoxetine hydrochloride did you use, capsule or liquid? Which solvent did you use to apply fluoxetine and D-limonene and what was your final application volume?
3. One of the limitations of the research is not researched of the dose-dependent effect. Manuscript can be added to the restrictions area.
4. BDNF and IL-6 results can be discussed in detail in the discussion section of the article.