Basically, this is a high-quality work, as it combines data detection and vector analysis of TCSPC, which can combine the advantages of both, not only beneficial for analyzing short-lived signals, but also able to obtain a system that fits well with the flow cytometry system, which is inspiring. My only comment is on the calculation of the upper limit of acquisition speed. according to the authors, the upper limit is about "100 cells per second, assuming a 50% cell transit duty cycle". In my understanding, the upper limit should be calculated according to Poisson distribution (see Cytometry Part A,71A, 10, 2007, Pages 783-796; doi.org/10.1002/cyto.a.20450).