In this manuscript, the authors developed a CNC/ICG/GOx/Cu/LAP gel for the locally treatment of colorectal cancer. The ICG was introduced to convert NIR light to heat and induce hyperthermia of tumor tissue, which also amplify the CDT effect; GOx was used for glucose deprivation as part of the ST strategy; and copper sulfate was introduced for hydroxyl radical generation and cellular glutathione (GSH) degradation in cancer cells. The writing is careless, in vitro and in vivo experiments were done haphazardly, and there are many problems should be solved.
1. Abstract, the statement of “An entangled gel structure comprising an organic nanorod (cellulose nanocrystal (CNC)) and inorganic nanodisk (Laponite (LAP)) was developed to perform a combination of starvation therapy (ST), chemodynamic therapy (CDT), and photothermal therapy (PTT) for localized
cancers.” is confusing, in fact, it is ICG/GOx/Cu-installed CNC/LAP gel for the local PTT/ST/CDT of cancer cells. In addition, what’s “copper(II) sulfate (Cu)”?
2. The gel is so complex, there are so many functional components including ICG, GOx, and CuSO4. However, only CNC/LAP, CNC/ICG/GOx/Cu/LAP, and CNC/ICG/GOx/Cu/LAP+NIR groups were carried on in vitro and in vivo experiments, it’s not enough.
3. Figure 6, where are the images and gel weights of ICG/GOx/Cu and CNC/ICG/GOx/Cu groups?
4. Does the NIR influence the Albumin, ALT, AST, and BUN levels in serum? The groups in systemic toxicity tests are very confusing. Does CNC/LAP not reflect the security of CNC or LAP?
5. Although the local injection of nano-sized delivery systems into the tumor tissue may alleviate the risk of systemic toxicities, it is not practical for clinical.
6. English usage should be improved substantially, there have been numerous grammar errors and inaccurate statements through the text.

The manuscript has been revised mostly according to the comments and suggestions of the reviewers, I recommend the acceptance of the manuscript for publication in Bioengineering & Translational Medicine.