Topic of the study has still actuality due to decreasing availability and increasing price of fish meal and increasing demand for novel feed ingredients. The aim of the work is well defined, composition and structure of the manuscript is logic, well-written. However, there are different issues in the paper that needs further major revision. Abstract- there is some inaccurate definition, which needs revision. LINE 20 dressing yield LINE 24 growth and nutrient utilization parameters

Keyword: please give other words than those are in the title

Introduction LINE 44 Catfish yields? production yield? please clarify LINE 47 the long run ? please rewrite the sentence LINE 63 please include the scientific name LINE 71-78 text moves before LINE 58 LINE 84 production and nutrient utilization parameters

Materials and methods Task 2.1 /2.4/2.5 should be merged into one task and shorten! There are repetition in the tasks. LINE 85 clear each Table 1. Digestible energy: please give the calculation LINE 118 similar questions LINE 150-153 please confirm W1 and W2. It is mean body weight in each calculation? If not, please use other letters. My suggestion is to leave only one parameters instead of three (for example weight gain (g)). LINE 153 please clarify the wet weight gain LINE 154 please clarify the weight gain (or this is similar with above mentioned?)

LINE 162 is missing the method for plasma separation LINE 172 Task 2.9 amd 2.10 should be merged and re-defined: Blood biochemistry

LINE 180 Please define fish body: whole body or eviscerated fish. It is missing the storage and preparation of the samples fro proximate analysis. LINE 197 Please use dressing yield.

RESULTS

General comment that there are too many tables and un-useful data. Table 6-10 should be merged into one table. Table 5 should be presented in Figure. There is not sense to present all the intermediate data calculated at the sampling times (W2, …W10). Is enough for most of the parameters to calculate only at final sampling, at the end of feeding trial, W12. What does it mean OVEARALL MEAN?

Figure 1,2,3 , Table 11-16. Please delete the data for Overall mean.

LINE 353 Repetition of the results (moisture and dry matter). LINE 359 Clear initial samples. Composition of the initial samples was not changed. rewrite the sentence. LINE 362 composition (%) is referring to dry matter or original matter? Please specify. LINE 368 Dressing yield LINE 383 concentration of chromic oxide is in dry matter or wet weight? Please clarify. TABLE 19 Please delete OVERALL MEAN. Or delete the mid-term values. TABLE 20 It has no sense to present mid-term data on digestibility coefficients. There are not find comments in the discussion part. Please delete or discuss.

Discussion LINE 451 Do not compare your data on blood chemistry with some nutritional trials using probiotics. Or rewrite the sense. LINE 468-470 similar problems. The non specific immune parameters may vary by using immunostimulant. Shrimp by product is better a protein source than immunostimulant. Please re-write you discussion regarding the immunological benefits of these protein sourses.

Reference

Most of the articles referred in the manuscript are older than 10-20 years. More new scientific papers must be included!