The title is descriptive the aim is clear but need to declare the novelty in the article references are relevant and up to date

The introduction is clear well constructed and informative

Overall the methods are well described, and they are valid for the study purpose
what are the PCR conditions used?

Results are well represented Figures and tables are clear and statistics were made Discussion need to be more specific and concentrate on discussing the obtained results and declare the novelty and the added value of the study

Overall the study is well constructed, and the aim is clear, but it only needs to declare what is new about this work compared to previous study in the same filed