Content of review 1, reviewed on April 22, 2016

Section 2.2, line 12 " pooled (by mass)" -- does "by mass" meaning normalized to equal masses of each input?

Table 1 -- Defined abundances have only single significant digit -- is this indicative of the precision of the mixing? The SMRT & ONT 2D are given to two significant figures -- is this appropriate? Table 3 gives the values to 3 significant figures .

Table 2 has 0 for the input abundance of the Klebsiella strains -- is this correct? If so, why was it detected in the community?

Are the methods appropriate to the aims of the study, are they well described, and are necessary controls included? If not, please specify what is required in your comments to the authors.

Yes.

Are the conclusions adequately supported by the data shown? If not, please explain in your comments to the authors.

Yes.

Does the manuscript adhere to the journal’s guidelines on minimum standards of reporting? If not, please specify what is required in your comments to the authors.

Yes.

Are you able to assess any statistics in the manuscript or would you recommend an additional statistical review? If an additional statistical review is recommended, please specify what aspects require further assessment in your comments to the editors.

There are no statistics in the manuscript.

Quality of written English

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Acceptable.

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References

    Chenhao, L., Rei, C. K., Hui, B. E. J., Qi, N. A. H., Andreas, W., Niranjan, N. 2016. INC-Seq: accurate single molecule reads using nanopore sequencing. GigaScience.