This is an interesting study, but is not a letter to the editor and as such needs the manuscript type changing which I will ask the editorial office to do

Figure 1 – Please define HFF in figure legend
Line 10 – minutes not mins
Line 11 – and the cell viability of the preparations was good, as determined…
Line 14 - new method is that it is..
Line 42 - tachyzoite not tachyzoites
Line 43 – method, the process
Line 45 – in vivo needs italicizing
Line 50 – remove excessive spaces
Line 54 – 8%
Line 58 – what do you mean by 5 volumes
Line 71, 74 – minutes
Line 86 – please include all supplementary material in the manuscript – ie figures
Please add in the headings of introduction, method, results and discussion