• Give a brief of cyto-ET and how the cells adhere. Can draw differences in its sample preparation.
• Polydimethylsiloxane: give a brief understanding of this coating to the nanofibers.
• Mention the ratios taken for the experimental set up and define how their combinations worked, therefore leading you to finalize one working ratio.
• What parameters were observed to prepare random and aligned fibers?
• Swelling and degradation analysis for the nanofibers to understand the stability of the structure can be incorporated.
• What mechanism is seen in the crosslinking process with the nanofibers.
• Can provide more insight to why the wells were created for cell culture and explain the significance of this procedure.
• Include quantitative analysis of the cellular response like cell migration and proliferation studies.

All the comments have been addressed