Content of review 1, reviewed on September 12, 2021
The manuscript from Maixner et al. describes a new method to improve ancient DNA recovery, through precipitation with linear polyacrylamide. The current version of the manuscript clearly describes all the methodological details needed to reproduce the work the authors describe. The conclusion are sufficiently supported by the evidence presented. The raw data generated with the experimental approach described should be made publicly available at the time of publication. A few minor comments follow:
Line 106
Suggeste change: replace "ancient bone and tissue samples", with: "ancient bone and soft tissue samples".
Line 129:
Replace: "The residual ethanol becomes removed", with: "The residual ethanol is removed".
Lines 150-51:
Replace: "until only a distinct translucent pellet remains left at the bottom of the tube", with: "until only a distinct translucent pellet remains at the bottom of the tube".
In some cases the authors use the measurement unit "ml" (for example at line 179) in other case they use the measurement unit "mL" (for example at line 179). They should consistently use only one of the two options throughout the entire text.
Line 209:
Start new paragraph:
"... described by Green and colleagues (Green & Sambrook 2016).
Beside the above... ".
Figure 2B: there is no indication of the mass of each band next to the bands in line M
Figure 2C: there is no description of the coloured dots
Source
© 2021 the Reviewer.
References
Frank, M., Christian, M., Y., J. H., S., S. M., Guido, V., Sebastian, L., Dario, P., Ildiko, S., Erika, M., Gyorgy, P., Ildiko, P., Giovanna, C., Albert, Z. 2022. Linear polyacrylamide is highly efficient in precipitating and purifying environmental and ancient DNA. Methods in Ecology and Evolution.
