Background and the knowledge gap : The trials of the pathogenesis of Ulcerative colitis (UC) as a type of inflammatory bowel disease (IBD), is ongoing to disclose the main involved etiology in this hardly treated disease . The pathology of the current disease was linked with different etiologies in different studies. In the first section of abstract is well defied to be clear but I think it needs a few modification in the last line of the purpose I quote " he aim of this study was to design a quantitative assay for detection of B. fragilis and ETBF and also to find their association with UC" I think it is appropriate to add a word of "bacteria " as : and also to find the bacterial association with UC because at the first glance may be misunderstood as the association is between the design of the quantitative assay and UC.

At the end of conclusion also it is hard to declare that this presumptive association at the level of the presence of understudy bacteria and rRNA with the development of UC.

The background is well written and organized but the novelty at the end of this section is not well mentioned and clarified according to the old and new studies.

In Methodology: the chosen of this type of 16S rRNA was not fluffily declared , to be chosen on what basis? Otherwise the methodology was well organized somewhat satisfiably described.

Figure1 legend poorly describes the red line straight slop, providing less appropriate amount of information regarding the measured values. I expect the legend to describe briefly experimental procedure, statistical values should be indicated.

The discussion I think it needs more attention by the authors regarding their presumptions and interpretation and finally the output of conclusion which on the other hand needs to justify the main results.