Reviewers’ Comments: Manuscript: CD147 Monoclonal Antibody Mediated Magnetothermally Responsive Nanocarriers / Doxorubicin for Active Targeted Hepatoma Thermo-chemotherapy

The research article by Li et al., explain the synergistic effects of CD147-MTRN/DOX, as a novel targeted nano-carrier for hepatocarcinoma treatment. The concept of this research work is very interesting and the representation of the experimental results need improvements. Before acceptance of the manuscript the following issues should be discussed and changed carefully.

1. In the “Abstract (METHODS)” section the authors mentioned “Lastly, its anticancer effects were evaluated by MTT”, Please rewrite the sentence with more clarity as MTT assay explained only the cytotoxicity.
2. In the “Materials and methods” section, please briefly mention about THF.
3. In the “Materials and methods” section, please incorporate the dialysis bag molecular weight Unit.
4. In the “Materials and methods” section, please give the full form of EDC and NHS
5. In the “Nanoparticles Aggregation in Tumors” section 4th paragraph instead of “the mice were killed” please use the term “euthanized”.
6. In “RESULTS AND DISCUSSION” section “Figure 1E, MS of CD147-MTRN/DOX was 30.6 emu/g, with superparamagnetic properties”, which is not correlate with the Figure 1E magnetic saturation curve. The Figure shows more than ~70 emu/g. Please correct.
7. In “RESULTS AND DISCUSSION” section from Figure 1E, authors claimed the nanoparticles are superparamagnetic behavior. How they confirm its superparamagnetic property. Please provide the coercivity and remanence data with magnetic saturation figure.
8. In Figure 4, please put the temperature scale instead of Low and High (Like Figure 5 temperature scale)