Dear Editor

Thank you for asking me to review this paper.

Major concerns

1. Paper need editing in its entirety by a native English speaker.

2. In figures, only left-hand polydactyly is evident in both families. Polydactyly has not been observed in right hands and feet. Therefore, the authors need to highlight this in the manuscript. Keeping in mind the left-right asymmetry and symmetry specific expression of genes, I would like the authors to check that expression of STKLD1 is symmetry specific or not.

Minor comments

1. Introduction, 2nd paragraph, replace ""cause for polydactyly"" with ""cause for limb deformity""

2. Mention unilaterality of the phenotype in upper limbs.

3. Describe any other skeletal abnormality, if exists.

4. Page 7, line 1, please correct gene name.

5. Discussion, first paragraph, correct please, ""play important role in the development of skeletal biology""

6. Page 7, second last line, ""ATP binding and nucleotide binding"". ATP is a nucleotide. Avoid repetition, please.

7. Page 7, last sentence, is there any sense of this sentence?

8. Discussion, last paragraph, first sentence, ""Polydactyly is a complex problem and unique challenge"". Please elaborate.

9. Please specify family A and B in the legends of Figure 1.