I have gone through the ms and observed that such an elaborate proposal for bio specimen collection from different animals had already been discussed in scheme of preservation of tissues for G10K Project in Journal of Heredity 2009:100(6):659–674. I remember this ambitious project of Steve O’Brien, Laboratory of Genomic Diversity at the National Cancer Institute,USA originally slated to be completed by 2015.

I do agree with the authors that the article aims to provide information which may help successful collection of large-scale data with significant utility for the researchers in the same field. However, such scheme of biological sample collection, without the support of the dataset will deprive readers for an assessment of its immediate impact therefore it would be rationally weak in its presentation for Giga Science.

Suggestions for authors:

Authors should have justified the efficacy of the methodology in terms of quality and quantity of the DNA recovered with the support of some data using the prescribed procedures. I therefore suggest this information will be worth publishing after the completion of the G10K Project. As the information presented here is in the form of suggestions only, which I fear may not be practicable in certain cases of DNA identification.

The large information about some of the most difficult bio specimen to work with has been published Michal R. Schweiger et al PlosOne May 2009 | Volume 4 | Issue 5 | 5548

Additional Remarks:

1. Language of the paper is explicable and attractive.
2. Information in ms is comprehensive and useful for the researchers in the same field.

Level of interest: Reject as not of sufficient priority to merit publishing in this journal

Quality of written English: Acceptable

Statistical review: No, the manuscript does not need to be seen by a statistician.

Declaration of competing interests: 'I declare that I have no competing interests'

(DR.I.HAQUE)