The manuscript entitled Design, synthesis and antitumor activity of novel pyrazolo[3,4-d]pyrimidine derivatives as EGFR-TK inhibitors showed detailed preparation of new pyrazolo[3,4-d]pyrimidine derivatives, characterization via different spectral tools in addition to anticancer activity screening. The title is specific and descriptive. The abstract items were included The references were cited well but some references are old The introduction part showed good description for the problem and also a clear description of the rationale The experimental part was written well The discussion part needs to be more concise and can compare the obtained results with related compounds. The conclusion part is a copy of the final part of the abstract and the authors do not refer to any future work as a continuation of this study.