The current study focuses on "Rapid Quantification of Pullulan in Fermentation Broth Using UV-Visible Spectroscopy and Partial Least Squares Regression". The work's concept is sensible and interesting; the article can be published with major modifications based on the suggestions below.
Comments:
The author should specify the study's aims in the introduction section rather than simply providing vague objectives. Is it better to write in points?
How many samples were used in this study? Please elaborate.
The abstract should provide information about which models produce the best results, rather than simply stating that PLS was utilized.
Have any preprocessing procedures been used? Spectral data taken from NIR always include background and unnecessary information.
Did the author validate the RPD values of the built models? If yes, should they be included in the manuscript?
Have you been able to regulate the temperature and humidity throughout the spectral data collection?
How many samples were used to do calibration, prediction, and validation?