The abstract is informative and structured and highlights the study objectives but It needs proofreading and grammar review. The title is informative and relevant References are Diverse and comprehensive recently published references

The introduction is informative and the research question highlight and justified for what is already known about the topic, but also but It needs proofreading and grammar review and need to use the active voice instead of passive voice for example "Microbiome is highly variable and a very important player mutually related to DM as well as obesity, e.g., weight gain may be also considered as a complication of insulin treatment [5]. Dietary intervention was found to modulate the gut microbiota and improve glucose control in individuals with T2DM [6].", There are many such examples throughout the research not just in the introduction.

Method: "All groups of mice, except the controls, received FED, which included 30 % of fats, 40 % proteins and 30 % carbohydrates" you should clarify this step if it can influence the results or not? The result is informative and It is very well presented and can be seen clearly through the tables and figures making it easier for the reader to understand the information.

Discussion and conclusion are informative and highlight and answer the aims of the study, also they supported by references and results, the limitations of this study are opportunities to inform future research. but also need proofreading and grammar review, and reducing of uses the passive voice.