The manuscript reviews the recent progress of supramolecular assemblies of small molecules (SASMs) and the biological functions. This manuscript introduced the most recent literature related to the development and biological applications of SASMs, including anticancer and antimicrobial therapy, sustain physiological functions of cells and exploration of cell behavior. At last, the authors discussed the further opportunities of SASMs from both the mechanisms of self-assembly and the chemical nature of compositions. The designed SASMs present a novel direction supramolecular chemical biology.
Overall, the context within this manuscript is well in order. We recommend the acceptance of this manuscript in RSC Chemical Biology after a minor revision which should improve the resolution of certain figures (for example, Figure 8a and Figure 9). It is not easy to read the chemical structures and texts.

The authors have addressed all the comments accordingly. Thus, we recommend the acceptance of this manuscript without further change.