Abstract, title and references

The aim of the study is clear and direct to the point, this study aims to find the co-relation between smoking and diabetic nephropathy and the role of curcumin as a therapeutic strategy.

I think this study is relevant and demonstrates the possible nephrotoxic effects of nicotine and Diabetes.

The title is clear and informative.

The Abstract is written in a simple and clear language, but abstract is long.

The references are relevant, current and cited correctly.

Introduction:

The introduction is clear and informative, it describes Diabetic nephropathy (DN), as well as, it demonstrated the damaging effect of smoking on the kidneys. Moreover, this introduction presents the potential role of curcumin as an anti-inflammatory and anti-oxidative compound.

Methods:

The study methods are valid, reliable and reproducible. The animal grouping and the used doses are appropriate, moreover the methods used were appropriate such as the biochemical analysis of kidney function, the molecular study using PCR, as well as, the histopathological study of (pancreas and kidneys).

Results:

The data are presented in a good manner. The results are presented in figures showing the levels of different parameters. The histological study is clear and descriptive. The results are statistically significant.

Discussion and Conclusions:

The discussion is written in a very clear and simplified order, the authors demonstrated the mechanisms through which curcumin protects against the combined oxidative stress of DM and NC exposure, in a simplified way. The molecular analysis was a great additive to the study. The conclusion is included in the discussion section but it would better to write the conclusion in a separate section.

Overall: The study design is clear and the article is consistent with itself.