The manuscript "Spatially-transformed fluorescence image data for ERK-MAPK and selected proteins within human epidermis" describes data of phospho-protein signaling pathway modulation during human keratinocyte differentiation. Based on immmunofluorescence labelling of Raf-1, MEK-1/2, ERK-1/2, but also calmodulin, β1-integrin, β4-integrin, stratifin, Jun-B, Fra2 and c-Fos, keratin 10 and 14, the sub-cellular and tissue distribution of each proteins was investigated.

Experimental methods and data collection are well described. Text from additional file 3 is useful to compare obtained data with the literature. Data show that expression patterns of ERKMAPK signaling proteins can be categorized into three groups: homogenous expression/distribution across the epidermis, asymmetric expression with strong signal either at basal keratinocytes or at epidermal keratinocytes.

However, data presented in additional file 3 panels C/D/E/F are not easy to grasp. The definition of "Dnorm" is not presented in this manuscript. The layer-normalization should be better described. Generally, I found the figures in the article hard to comprehend. In fact, to better understand this data note, I had to get information from a recently published publication from the same authors (doi:10.1186/s12918-015-0187-6) which is cited several times. However, a reference does not substitute for basic clarity. I suggest that the authors better guide their readers throughout the manuscript so that they do not need to rely too much on previously published material.

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Please indicate how interesting you found the manuscript: An article of importance in its field

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