Dear authors, I read your manuscript titled "Mutation of brain aromatase impairs behavior and neuroplasticity in adult zebrafish" with interest. The present manuscript studies the role of brain aromatase in neurobiological regulation of diverse behaviors as well as its influence on brain proliferation and dopaminergic and serotonergic neurotransmission. I recommend that the manuscript be published after minor revisions. I have some suggestions/comments/questions that may contribute to the work:

Introduction: The authors must justify choosing zebrafish as a model organism.

page 6: "The zebrafish were maintained under standard conditions of housing, with a photoperiod of 14:10 light:dark. " - More information would be useful for readers.

• It would also be interesting to study oxidative stress enzymes such as SOD, CAT or GPx.

• No mention is made of whether power analysis was conducted to determine sample size. Was this number determined based on prior experiments or statistical power?