The title is suitable for work and the summary is well written. In the introduction, the authors did not review the rest of the members of the HKT family such as HKT2;3 and HKT2,1 which contributes with the HKT1;4 and HKT1;5 genes on the QTL site to salt stress tolerance in wheat plant.

To improve this article: 1. For each firm conviction related to a new function of this gene, the results require the use of qRT-PCR technology or GUS technique, to determine the gene expression of the target gene, which means it is not limited to using two-electrode voltage-clamp technique (electrophysiology)technique.

1. I think it would have been better to use more than one wheat cultivar for comparison first and then the results are more accurate.

2. Also, I think it was better to test other ions such as Mn or Mg besides (K+, Li+, Cs+ and Rb+) due to the close physiological relationship between sodium and potassium in case of salt stress tolerance.

It is possible to increase the concentration of NaCl more than 3mM in order to test the permeability of the Xenopus oocytes membrane to achieve an accurate comparison of ions (K+, Li+, Cs+ and Rb+).

In the discussion, the authors focused a lot on HKT family members in rice plant, while it was better to review and discuss previous work related to HKT family members in wheat, in doing so, it need to be strengthened. I think it is certainly adhering to those comments and notes for confirm the results of new discovery relates to the function relates to the function of HKT family's genes in order to improve the tolerance of salt stress in the wheat crop.