In this manuscript, the authors designed the first in-vitro model involving uniaxialmechanical stress applied to aligned human primary muscle-derived cells, using a biocompatible (OlPHM) covalently attached to a stretchable PDMS support. However, there are still some problems need to be considered. Thus, I recommend the authors revising the following queries.
1. The manuscript presents a lack of characterization of the tensile properties of materials. Author should improve and modify it.
2. Author should increase testing of the stability of the coating.
3. The conclusion section of the article is insufficient. It should include comparisons with other existing technologies, their shortcomings, and future developments.
4. It is suggested that the author explore the changes in wettability of the material after coating and conduct contact angle testing.
5. In Figures 2c and d, there are too few data points and the fitted value R2 is lower than 0.99, which is not convincing enough.
6. There are other good articles on chip. The authors are suggested to read them and use them as references. Following article may be mentioned: Smart Med, 2024, 3:e20240009; 2023, 2:e20220030; 2023, 2:e20220010.

All comments have been addressed.