The paper “Rapid Quantification of Pullulan in Fermentation Broth sing UV-Visible Spectroscopy and Partial Least Squares Regression” provides a useful method, to monitor Pullulan in Fermentation Broth using UV-VIS that can be used as an alternative to ATR-FTIR, HPLC-MS, and colorimetric methods used for the same purpose. The reliable results, a concise and clear description, great versatility and easiness of implementation of the method make the manuscript suitable for publication in Analytical Methods.
There are just a few minor points to address:
Please specify in section 2.3 that the dimensions of the PDW set are 80x197
In Tables and Figures indicate the units of the RMSEs
It could be useful to include a univariate calibration at the best wavelength and included it it the supplementary material just for comparison.
Please indicate the decision-making process that allows the choice of the parameters reported in Table 1.
The Parity plot in Figure S2 presents high dispersion at high concentrations which is not observed in Figure S1. Please discuss.
It is not clear to me in section 3.4 if the reference gravimetric measurements used to compare models is the same as those used to construct the models.
In table 4 the LODs (g/L) are very high in comparison to those reported by NIR and UV (mg/L). Please indicate the suitability of the method based on the intended purpose.

As all the reviewers' questions were satisfactorily addressed, the manuscript is now suitable for publication.