In their study, Buaron et al. investigate the application of low-frequency ultrasound (LFUS) in disrupting brain tumor structures to inhibit tumor growth. Their findings demonstrate promising potential; however, several significant concerns warrant attention before publication.
1. In the in vitro cell availability assay, it is essential for the authors to assess the safety of LFUS on normal brain cell lines.
2. The investigation into tumor growth should encompass an examination for potential metastasis in both intracranial and extracranial organs. Long-term observations, especially extending to 1-2 weeks post LFUS treatment, are recommended.
3. Comprehensive safety evaluation in treated animals is crucial. Additionally, quantitative safety assessments, particularly those pertaining to the findings in Figure 3, would enhance the manuscript's depth and clarity.