Here the authors analysed pncA mutations and their effects on pyrazinamidase (PncA) activity. The objective was
1) to increase the knowledge of mutations in the pncA gene related to pyrazinamide (PZA) resistance, and 2) to facilitate rapid molecular diagnosis of PZA resistance in M. tuberculosis. The pncA gene was sequenced and analysed in 465 clinical isolates. 30 new mutations were identified. The pncA activities of the 30 new mutations were tested using a pncA gene knockout Escherichia coli strain to avoid the lack of reproducibility of PZA MIC tests at acidic pH.

I have no conflicts of interest.