Abstract, title and references

Use of Trichoderma species as biological control agents against plant disease is well studied for many decades; in fact, i don’t saw any novelty in this study.

The abstract is written in a clear and detailed way, but there is a lot of plus detail (Four initially lines by the abstract).

Title is written by well structured; however you must insert the country name instead of the Garlic and Onion due to, it is international paper.

References used are written correctly and have been characterized by modernity as well as being related by completely to the main topic.

Comments on introduction/background

The introduction provided appropriate details of the topic, as well as the reason and justification it where, noted, the study aim to identification at a species level to Trichoderma isolated from rhizosphere of garlic and onion, and Antagonistic activity test of isolates against S. cepivorum.

but, Details of the Trichoderma role in biological control taken a large part of the introduction compared to the benefit and role of the molecular identification of species, in addition to elaborating on the role of Trichoderma in the produce antifungal metabolites, release hydrolytic enzymes, and their mycoparasitic behaviour, as well as the production of other substances that enhance plant growth that were not included in the study.

It was better, if isolates tested against the pathogen first and then the molecular identification performed to isolates which gave only a high antagonism.

Comments on methodology

The researcher did not explain how and where he got five isolates of Trichoderma spp.( and/or reference). Depending the researcher to only five isolates is not clear. Does this mean soil was previously treated with these isolates? Why many bacteria strains and other fungi present in the soil were neglected that may have had a major role also in protecting onions and garlic from plant diseases?

Was researcher had to use several methods in testing the antagonistic ability of the Trichoderma isolates against pathogen in vitro such as culture filtrate and volatile compounds technique, and it is not limited to antagonism only.

Defining of variables using five isolates only are not appropriately to represent onion and garlic fields.

Comments on data and results

In fact, explanation and scheduling of the results were well showed but at the same time, the results are considered inaccurate unless the experimental units are repeated at least three times, after which they are statistically analyzed.

Some results are from best insert it as photo in the text such as Antagonism test in petri dish.

Comments on discussion and conclusions

The discussion part is written well except the last part in the paragraph (Reproduction Tests for every isolate) is no found scientific explanation of results through discussed with the other citations. Conclusions: The conclusions have given completely answers to the topic aims but it need to support by using the references.

In addition, this study not considered are limitations where, there several opportunities to inform future research.