This works focusses on the dispersal patterns of non-indigenous ascidians larvae in the Eastern Mediterranean combining biophysical simulations and observations/presences of the species. This comprehensive approach rises the overall interest and robustness of the work. Furthermore, the region is a hot spot regarding recent warming and invasive species expansion.
The paper is well written and biological issues like PLD or spawning seasons are well supported.
However, simulation details are really scarce and is not easy to follow eg. the number of simulations carried out, is it only a single numerical experiment per species? Are specific spawning seasons used per particles release? Is it not clear in the ms.
Most of the model information relies on a preprint and should be included here at least within Supplementary Information.
I do not see a proper link of the spawning season projections with the rest of the paper. Particularly if these spawning seasons are not applied in the release of the particles.
I could not suggest the publication of the paper at this current state but after a major revision the ms could be deeply improved.

The authors have satisfactorily addressed the issues raised. At this point, I recommend the publication of this work