The article provides a clear aim and method of study. However, the degree of cross-reactivity between ochratoxin A and B should include, because it influences the IC50, LOD, and recovery of immunoassays. The significant number of recoveries might be over accuracy regarding the machines used. 1. In the introduction section, there are many immunochromatographic assays for the detection of ochratoxin A. please provide the gap of knowledge for this study. 2. demonstration of the research question can enhance the objectives of this study “a monoclonal antibody against ochratoxins (OTA and OTB) with high affinity, and to establish sensitive ic-ELISA,” Thus the sensitivity of the previous immunoassay should provide. 3. what is the binding characteristics of mAb preferred, which can improve the immunoassay performances, and why? 4. In the method section, in the process of ELISA, usually Na2CO3 and NaHCO3 was mixed as buffer pH9.6. In this article 0.05 M Na2CO3, please explain how to achieve pH9.6. 5. The chemical composition of the blocking buffer should be detailed. 6. The concentration of tween in the washing buffer should indicate. 7. The generation of mAb in ascites should be indicated in the improved procedure. 8. The procedure for the evaluation of affinity was not clear. The information provides only the procedure of cross-reactivity. The preparation of the sample should be described. 9. The definition of titter used in this study should be informed because figure 2A did not show the name of the y-axis. 10. please explain why you select the only clone 6E5 11. Figure 3B and C should use the same name as the y-axis, and the font size should be consistent. 12. legend of figure 3B should be clearly demonstrated. 13. the yield of purified mAb should provide in the results. 14. Figure 4B, usually, X-axis, should be log scale, which provides the log-lin linear regression. 15. The affinity of OTB should be tested. 16. the detection limit in the real sample should be provided. 17. the discussion of performance between Colloidal Gold Strip and Gold Nanoflowers (AuNFs) should provide in deep. 18. overall, the research developed an effective and simples method for analysis. After a major revision, the article should contribute to the impact on the field of food analysis.