The author has explained the objectives of the study perspicuously about role of TXNIP that relays a signal to mediate between ER stress and NLRP3 inflammasome activation in PE. The author did the investigation accordingly where they assess the cells transfected with TXNIP /TXNIP siRNA to see the effect together with external inducer/inhibitor of ER stress as the control. The references used are appropriately cited by the author and the title is relevant to the abstract. This study might give new insights in regards of ER Stress and its relationship with preeclampsia for medical therapeutic strategies. However, some minor improvements are described below:

Introduction The background study is sufficiently explained. However, the references number 11-14 could be improved by referring to primary study for UPR/ER stress by Walter/Mori/Reiling et al. The research question is well outlined to search of connection between ER stress - TXNIP - NLRP3 inflammation which lead to PE.

Materials and Method The sample population employed by the author is appropriate and the variables defined well in the study. The methods are valid for the study and enough details of methods were presented to clarify the experimental works.

Results The author has explained the results succinctly. Some improvements can be made as follows:

1. The sample population participated in the study should be characterized to differentiate between normal and with PE
2. Fig 1a, the author should include the percentage of cells that express ASC, TXNIP and NLRP3.
3. Also, Fig. 3d, the author should include the percentage of cells expressing IL-1B for all samples
4. In section 3.3, last sentences, the author mentioned about "the levels of NLRP3's downstream target was increased". The clarification is somewhat simplified with the results. It is best if the author mentioned about the TXNIP siRNA vs control/(H/R)/TM as well as localization of stain to ease the reader to observe the image. In addition, it seems the staining of DAPI for H/R+TXNIP siRNA and control (slightly) were under-exposure compared to H/R and TM sample. It could instigate a manipulation if the exposure is too much different. Please clarify if there were hurdles of experimental technique or if possible, please include image of DAPI with similar exposure across 4 variables sample.
5. If possible, please include the confident level of results in Fig. 3c since this is the primary results for the hypothesis presented.

Discussion The findings has been discussed thoroughly by the author. The conclusion from the discussion has complemented the aims of the study and further works are recommended in this section. It is suggested that the author could discuss the limitations in the experimental works to brief the reader the obstacles endured in the study.