The title and abstract cover the main aspect of the research The introduction provides relevant background dan information to the study The Method is quite clear but needs some information - The methods section ‘chemicals’ should be written as chemicals and equipment as it includes glucose estimation kit and the cholesterol and LDL estimation kits - Please state the software used for the statistical analysis

The result and discussion part shows the findings correlate with the results but some need improvement The manuscript is well written and there are no grammatical errors. -The unit used to show the centrifuge speed ‘4000g’is wrong