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Abstract

The rapid spread of coronavirus SARS-CoV-2 greatly threatens global public health but no prophylactic vaccine is available. Here, we report the generation of a replication-incompetent recombinant serotype 5 adenovirus, Ad5-S-nb2, carrying a codon-optimized gene encoding Spike protein (S). In mice and rhesus macaques, intramuscular injection with Ad5-S-nb2 elicits systemic S-specific antibody and cell-mediated immune (CMI) responses. Intranasal inoculation elicits both systemic and pulmonary antibody responses but weaker CMI response. At 30 days after a single vaccination with Ad5-S-nb2 either intramuscularly or intranasally, macaques are protected against SARS-CoV-2 challenge. A subsequent challenge reveals that macaques vaccinated with a 10-fold lower vaccine dosage (1x10(10) viral particles) are also protected, demonstrating the effectiveness of Ad5-S-nb2 and the possibility of offering more vaccine dosages within a shorter timeframe. Thus, Ad5-S-nb2 is a promising candidate vaccine and warrants further clinical evaluation. A vaccine protecting from SARS-CoV-2 infection is needed. Here the authors generate a replication-incompetent adenovirus based vaccine expressing SARS-CoV-2 spike, show protection from infection in non-human primates, and analyze the immune response after intramuscular and intranasal vaccination.

Authors

Feng, Liqiang;  Wang, Qian;  Shan, Chao;  Yang, Chenchen;  Feng, Ying;  Wu, Jia;  Liu, Xiaolin;  Zhou, Yiwu;  Jiang, Rendi;  Hu, Peiyu;  Liu, Xinglong;  Zhang, Fan;  Li, Pingchao;  Niu, Xuefeng;  Liu, Yichu;  Zheng, Xuehua;  Luo, Jia;  Sun, Jing;  Gu, Yingying;  Liu, Bo;  Xu, Yongcun;  Li, Chufang;  Pan, Weiqi;  Zhao, Jincun;  Ke, Changwen;  Chen, Xinwen;  Xu, Tao;  Zhong, Nanshan;  Guan, Suhua;  Yuan, Zhiming;  Chen, Ling

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  • Brief overview of the paper and its main findings

    Describes the results of an adenovirus-vectored COVID-19 vaccine test in animals. Claims that vaccine confers protection from SARS-COV-2 challenge in rhesus macaques.

    Major and minor points

    They have a fundamental design safety flaw. They will cause IgE mediated sensitization to adenovirus proteins. So otherwise harmless adenovirus infections will now become life-threatening. This is exactly the same problem as COVID-19 where harmless coronavirus (CV) has become life-threatening due to IgE mediated sensitization using dirty, CV protein contaminated, infected animal derived vaccines.

    That is why allergy medications such as histamine H1/H2 blockers (cetirizine/famotidine) help in COVID-19.

    Immunological mechanisms explaining the role of IgE, mast cells, histamine, elevating ferritin, IL-6, D-dimer, VEGF levels in COVID-19 and dengue, potential treatments such as mast cell stabilizers, antihistamines, Vitamin C, hydroxychloroquine, ivermectin and azithromycin

    https://doi.org/10.5281/zenodo.3748303

    As I have been predicting for 7+ months, there is now evidence that Famotidine (antihistamine, H2-blocker) helps in COVID-19. Study below. The mechanism involved is explained above.

    Famotidine Use is Associated with Improved Clinical Outcomes in Hospitalized COVID-19 Patients: A Retrospective Cohort Study

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242191/

    They need to publish EVERY contaminant in their vaccine and design FMEA.

    The contaminated Wuhan lab created the SARS-CoV-2 virus by accident. Same contamination affects vaccine safety.

    Root cause of COVID-19? Biotechnology's dirty secret: Contamination. Bioinformatics evidence demonstrates that SARS-CoV-2 was created in a laboratory, unlikely to be a bioweapon but most likely a result of sloppy experiments https://doi.org/10.5281/zenodo.3766462

    Why do they think the vaccine will not fail the same way as Dengvaxia did?

    Irrational dengue vaccine designs that ignore IgE and IgG4 mediated effects are destined to follow in Dengvaxia's disastrous direction? https://doi.org/10.5281/zenodo.1476291

    Example of the type of homework they need to do before making ANY claims of vaccine safety.

    ERVEBO Ebola vaccine will create a rice allergy epidemic, add to numerous autoimmune diseases, cancer and make Ebola disease even more severe. Design for safety and vaccine safety regulation remain abject failures. Incompetence or indifference? https://doi.org/10.5281/zenodo.3595020

    Conflicts of interest

    None

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