Research has shown that growing up in an environment in which emotions are invalidated (i.e., ignored or responded to negatively) by parents is associated with later difficulties regulating emotions. Meanwhile, dispositional mindfulness has been shown to engender a greater capacity for emotion regulation, through use of adaptive strategies like cognitive reframing and minimizing use of maladaptive strategies like expressive suppression. The current study aimed to explore the role of invalidating childhood environments on use of cognitive reappraisal and expressive suppression as emotion regulation skills and to investigate the role of mindfulness in this relationship. Participants were recruited via Amazon Mechanical Turk (n = 1094,M-age = 58.3% women) and completed self-report measures assessing perceptions of maternal invalidation, mindfulness, and emotion regulation. Results demonstrated that the mindfulness facets of describing, non-judging, and non-reactivity partially mediated the relationship between perception of maternal invalidation and expressive suppression. Awareness and non-reactivity were found to mediate the relationship between perception of maternal invalidation and cognitive reappraisal. Clinical implications are discussed.