A type of polymeric nanoparticles loading indocyanine green and Pt(II)-porphyrins (ICG-Pt-NPs) is constructed to achieve a synergistic effect of combined photothermal and two-photon activated photodynamic therapy. The nanoparticle core comprises the photosensitizer Pt(II)-porphyrins (PtTFPP), and organic semiconducting polymer (PFO) that acts as a two-photon antenna. Negative ICG molecules, an NIR-absorbing photothermal dye, can be loaded into the positively charged poly-L-lysine (PLL) shell of the polymeric nanoparticles via electrostatic interaction. In these carefully designed ICG-PtTFPP integrated nanoparticles, PtTFPP absorbs the photonic energy transferred by the PFO polymer under two-photon laser excitation at 740 nm to induce photodynamic cancer cell death, while ICG offers nanoparticles a strong photothermal performance under 808 nm laser irradiation. Compared with photodynamic therapy or photothermal therapy alone, the combined therapy had a significantly synergistic effect and improved the therapeutic efficacy with near-infrared irradiation.